Description
The first part of the NeurotechEU summer school will be held on-line and theoretical background of sample preparation and Electron Microscopy techniques will be discussed by tutors from University of Debrecen (UD) and partners from the NeurotechEU.
The second part of the course will be a 5 day long practical course, held in the Electron Microscopy Laboratory of the Department of Anatomy, Histology and Embryology (UD). During the hands-on training participants will have the chance to observe fixation methods, sectioning with the ultramicrotome, osmification, dehydration, embedding, mounting. A separate day will be dedicated to manual and automated (ATUMtome) serial ultrathin section cutting. They will also get an introduction to recognizing basic neuronal and glial structures on EM images.
Host university teaching staff /experts: Greta Kis, Vera Etelka Szarvas and Peter Szucs
Number of ECTS granted: 3 ECTS
How does the physical program look like?
1st day (17 July, Monday)
Transcardial perfusion, brain tissue sampling / vibratome sectioning (for SBF-SEM)
2nd day (18 July, Tuesday)
Embedding of resin impregnated tissue blocks in capsules / mounting of resin impregnated sections on slides / preparation of formvar coated grids
3rd day (19 July, Wednesday)
Practical overview of osmification, dehydration and embedding steps
4th day (20 July, Thursday)
Manual and automated cutting of serial ultrathin sections using ultramicrotome and ATUMtome / glueing osmificated blocks on pins for SBF-SEM / Sightseeing (optional)
5th day (21 July, Friday)
Manual and automated cutting of serial ultrathin sections using ultramicrotome and ATUMtome / glueing osmificated blocks on pins for SBF-SEM / Sightseeing (optional)
Profile of participants
Students enrolled in universities part of the NeurotechEU alliance, electron microscopy technicians, laboratory assistants.
In order to apply, you must be a student of the following degree programs of the University of Bonn:
- MSc./PhD Neurosciences
- BSc./MSc./PhD. Psychology
- BSc. Molecular Biomedicine, M.Sc. Biochemistry, M.Sc. Immunobiology + PhD students